Entwicklung und Anwendungen von Biokeramik

Effects of Si(IV) Released from Chitosan-Silicate Hybrids on Proliferation and Differentiation of MG63 Osteoblast Cells

Abstract

Y. Shirosaki, K. Tsuru, S. Hayakawa, Y. Nakamura, I. R. Gibson and A. Osaka

Chitosan-?-glycidoxypropyltrimethoxysilane (GPTMS)-tetraethoxysilane (TEOS) hybrid membranes were prepared by a sol-gel method. Effects of Si(IV) released from them on proliferation and differentiation were examined in terms of cell metabolic activity and alkaline phosphatase (ALP) activity of MG63 osteoblastic cells. The amount of Si(IV) released from the hybrid membranes increased with the TEOS content. Released Si(IV) inhibited cell proliferation, while it promoted cell differentiation. Thus, osteocompatibility of the chitosan hybrid membranes can be controlled due to the amount of Si(IV) released from them as the hybrids in the system chitosan-GPTMS-TEOS are applied for cell culture.