Biosensors & Bioelectronics

Coding by Different Diameter Impedance, Detection Method of Microspheres Which are Used to Produce Liquid Biochip

Abstract

Zhanke Wang, Tian Hu, Xiaolu Hu, Tao Song, Wangshen Lei, Honghua Zhou, Xiao Yan, Hong Dai and Jin Chang

This research was aiming at developing liquid biochip which was coded by impedance microsphere of different diameter and providing research foundation for its detecting device. Adopting the method of loading constant current source between both ends of micropore in ruby, it were tested that three sorts of microspheres’ impedance with particle sizes of 2μm, 5μm, 10μm. It was resultantly found that microsphere diameter is in direct proportion to impedance and voltage between both ends of micropore. In other words, microsphere of different size could be distinguished from each other by their impedance. Our research drew the indication that impedance of microspheres in different sizes could be theoretically calculated as well as measured and automatically distinguished, offering experimental and theoretical foundation for liquid biochip development.