Entwicklung und Anwendungen von Biokeramik

BSA Attachment on Apatite Surface Modified with Zn2+ and Sr2+

Abstract

E. Mavropoulos, M. L. F. M. Kede, N. C. C. da Rocha, A. Machado Costa, A. Tosi, M. H. Prado da Silva and A. M. Rossi

Synthetic hydroxyapatite was modified with zinc and strontium by two methods: ion exchange and coprecipitation synthesis. Hydroxyapatite and metal modified hydroxyapatite samples were characterized by XRD, ICP-OES, and FTIR-DRS. BSA adsorption experiments were accomplished during 24 hours using 1.0mg/mL of protein. UV spectrometry was used to quantify the protein at 278 nm. The results suggest that metal presence on the surface or in the bulk of hydroxyapatite improves the protein adsorption efficiency. FTIR-DRS showed that the protein main secondary structure ?-Helix is involved in the adsorption process.