Molekulare und genetische Medizin

An Alternative Test Procedure for the Protective Efficacy of Brucella Vaccines

Abstract

Zhen Wang, Peng F Bie, Tong L, Wu and Qing M Wu

Administration of live Brucella vaccine is required to prevent the spreadof ruminant brucellosisin affected livestock herds. This study optimized the mouse model to test the protective efficacy of live Brucella vaccine. To optimize the protective efficacy test procedure, the absolute infective doses were determined in mice as 10 Colony Forming Units (CFU)/mouse for B. melitensis 16M and 50 CFU/mouse for B. abortus 2308, which were then used as the challenge doses for the respective strains. The optimal vaccination doses of vaccine Brucellasuis S2 in mice were 102.25 CFU and 103.5 CFU/mouse, which could confer ≥ 80% protection in mice against challenge by B. melitensis 16M or B. abortus 2308, respectively. In addition, challenge with B. melitensis 16M or B. abortus 2308 should occur just at 3.63 weeks and 4.75 weeks post-inoculation, respectively. The protective efficacy test not only was more accurate and took less time but also was consistent with the evaluation index in host animals. Our study indicated that the mouse model could be used to test the protective efficacy of live Brucella vaccines during their production and development.