Entwicklung und Anwendungen von Biokeramik

A New Synthetic Diet Increased Bone Mineral Density and Strength of Ovariectomized Rat

Abstract

Hiroshi Nakada, Takehiro Watanabe, Takahiro Takahashi, Kanami Fujita, Yasuhiro Tanimoto, Mari Teranishi, Takao Kato, Yasuhiko Kawai, Toshiro Sakae and Racquel Z LeGeros

This study aimed to determine changes in Bone Mineral Density (BMD) and bone strength of ovariectomized rats fed a synthetic diet of Mg, Zn, and F incorporated with carbonate apatite. Group 1 (n=4) was fed a Normal Mineral Diet (NMD) and Group 2 (n=4) was fed the Synthetic Diet (SD: 0.74% Ca, 0.48% P, 0.10% Mg, 365.4 ppm Zn, 51.01 ppm F and 0.27% CO3). Rats were sacrificed after eight weeks and the femur was isolated. The micro-CT results showed that values of BMD for Group 2 were higher than those of Group 1. Bone strength values of Group 2 measured by a 3-point bending test were significantly higher than those of Group 1. These results indicated that Group 2 stimulated bone quality, increased BMD and bone strength. The SD has the potential to prevent bone loss and as an osteoporosis therapy.