Tian Liang and Reena Roy
The goal of this research was to quantitatively measure the amount of salivary amylase in a sample by using UVVIS and the SALIgAE® reagent. The samples included serial dilutions of standard human salivary amylase, human saliva, as well as other body fluids from human and animals. These were added to the SALIgAE® reagent and the absorbances of the solutions were monitored by the UV-VIS at 403 nm every 30 seconds over a 10 minute period. The lowest concentration of salivary amylase detected by UV-VIS was 1 ppm (parts per million), or 0.0010 units of amylase, while the color of the reagent was clear upon visual observation at this dilution. The absorbance change and kinetics of the reaction between the amylase and the SALIgAE® reagent were determined to be proportional to the concentration of the salivary amylase in a dilution. This relationship can be used to quantify the amount of amylase from items of evidence.
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