Veterinärmedizinische Wissenschaft und Technologie

Haematological and Histopathological Vicissitudes Following Oral Inoculation of Graded Doses of Pasteurella multocida Type B: 2 and its Lipopolysaccharide in Mice

Abstract

Omar Suwaidan Ali, Lawan Adamu, Faez Firdaus Jesse Abdullah, Yusuf Abba, Hazilawati Binti Hamzah, Mohd-Azmi ML, Abdul Wahid Haron and Mohd Zamri-Saad

Pasteurella multocida type B: 2 is responsible for major animal diseases of economic importance in both developed and developing countries. Haemorrhagic Septicaemia (HS) could inflict devastating effects on blood tissues and organs in the host animal. Therefore, the current study aims to investigate the haematological and clinico- pathological responses in mice following oral inoculation of graded doses of Pasteurella multocida type B: 2 and its lipopolysaccharide. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n=10) and these were inoculated with 0.4 ml of PBS pH 7.4 orally. The treatment groups (B; n=25 and C; n=25) were inoculated with P. multocida type B: 2 and its lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B101, B103, B105, B107 and B109 for Pasteurella multocida and C101, C103, C105, C107 and C109 for LPS respectively. The mice were observed for 120 hours post- inoculation. The clinical signs (Ruffled fur, Ocular discharges, Level of alertness and Laboured breathing) were significantly different (p<0.001) in mice inoculated orally with variable doses of Pasteurella multocida type B: 2 and its LPS. RBC, PCV, haemoglobin concentrations, PT, APTT, Thrombocyte, WBC, Lymphocytes, monocytes, plasma proteins, band and segmented neutrophils were significantly different (p<0.0001) in mice inoculated with graded doses of Pasteurella multocida type B: 2 and its LPS. Inflammatory cells, degeneration, necrosis and congestion were significantly different (p<0.0001) in mice inoculated with graded doses of both Pasteurella multocida type B: 2 and its LPS. In conclusion, 109 cfu of Pasteurella multocida type B: 2 and its lipopolysaccharide have devastating effects on organs and blood tissues.