Zeitschrift für Zytologie und Histologie

Correlative Study of Imprint and Crush Cytology with Histopathology in Endobronchial Growths

Abstract

Gulam Qadir Bhat, Showkat Ali Mufti, Thussu S Kumar, Sonaullah Shah, Parviaz Ahmed Koul, Parveen Shah, Ather Hafiz Khan and Rafi Ahmed Jan

Objective: This study was undertaken to study the correlation between imprint cytology, crush smears
and histopathology in endobronchial growths using fiberoptic bronchoscopy.
Methods: The study was conducted in the Department of Internal Medicine and Pathology at Sher-i-Kashmir
Institute of Medical Sciences. Eighty-one patients with clinical and radiological evidences of lung lesions were
enrolled. From all patients five pieces of tissue were obtained during fiberoptic broncoscopy which revealed an
endobronchial growth. From each bit of tissue an imprint and crush smear were prepared by imprinting the tissue on
a clean surface of glass slide without compressing the tissue while as crush smear was made by gently crushing the
tissue between two glass slides. The tissue specimens were then placed in formaldehyde solution for histological
examination. Standard statistical methods were used to analyze the data (chi-square (χ2) analysis; a Fisher’s
exact test was used to evaluate the data if more than 25% of the expected values were <5). The level of significance
selected was P<0.05.