Zeitschrift für Zytologie und Histologie

Correlation between Id Genes Expressions and Histological Grade, Sonographic Findings in Breast Cancer

Abstract

Huijie Shi, Lei Zhang, Yanjun Qu, Lifang Hou, Ling Wang and Min Zheng

Objective: To investigate the expression significance of inhibitor of DNA binding (Id)-1, Id-2 and Id-3 mRNAs in breast cancer patients, and explore the correlation between sonographic characteristics, histological grades and expressions of Id-1, Id-2 and Id-3 mRNAs.
Methods: The expressions of Id1-3 mRNAs in 62 breast cancer patients and 49 normal controls were examined by using real-time fluorescent quantitative reverse transcription polymerase chain reaction (RT-FQ-PCR). The correlations between sonographic characteristics, histological grades and expressions of Id1-3 mRNAs were analyzed with one-way ANOVA.
Results: Id-1 and Id-3 mRNA were higher expressed in breast cancer patients than those in normal control cases (Id-1: 0.0141 ± 0.01025 versus 0.0075 ± 0.00309, p=0.0001; Id-3: 0.0139 ± 0.01029 versus 0.0078 ± 0.00312, p=0.005), while expression of Id-2 mRNA was lower (Id-2: 0.0195 ± 0.01299 versus 0.0275 ± 0.01401, p=0.033). Along with the increase in histologic grades, Id-1 and Id-3 mRNA expressions in breast cancer tissue showed an increase tendency, however, Id-2 showed an opposite expression pattern with Id-1 and Id-3. The expression of Id-1 was higher in patients with keloid-like and hyperechoic halo, while Id-3 mRNA was highly expressed in patients with keloid-like and peripheral catheter expansion under ultrasonography.
Conclusion: Expression levels of Id-1 to 3 mRNAs are highly correlated with histological grades and sonographic characteristics of breast cancer.