Krebswissenschaft und -therapie

1205Lu is Human Melanoma Depending on the Source

Abstract

Patricia Brafford, Katrin Sproesser, Clemens Krepler and Meenhard Herlyn

1205Lu is a “work horse” melanoma cell line established in our laboratory at the Wistar Institute. It has been used in countless studies since its derivation in 1993. The cell line was generated by sequentially passaging the primary human melanoma cell line WM793 through immune deficient mice and harvesting lung metastases. The lungs from the fifth passage were cultured to establish the permanent cell line 1205Lu. Due to the many requests for melanoma cell lines and in an effort to facilitate distribution of the Wistar Melanoma Cell Lines, Coriell Institute of Medical Research (Camden, NJ) was contracted to grow and distribute our most popular melanoma cell lines including 1205Lu, which was submitted to Coriell in 2008. Before sending each cell line stock to Coriell, we tested for mycoplasma and short tandem repeat (STR) profile using AmpFlSTR® Identifiler® PCR Amplification Kit (Catalog Number 4322288) by Life Technologies